Δημοσιεύσεις
Δημοσιεύσεις
2015 |
Lantzouraki, D. Z.; Sinanoglou, V. J.; Proestos, C.; Zoumpoulakis, P. Total phenolic content, antioxidant capacity and phytochemical profiling of grape and pomegranate wines Journal Article In: RSC Advances, vol. 5, iss. 123, pp. 101683- 101692, 2015, ((I F: 3.84)). Abstract | Links | BibTeX | Ετικέτες: grape, phenolic content, phytochemical profiling, pomegranate @article{Lantzouraki2015c, The object of this study was to determine the phenolic profile, the total phenolic content and antioxidant capacity of pomegranate wine and compare to multi-varietal red wine using different spectrophotometric and spectrometric techniques. Total phenolic content was determined by the Folin–Ciocalteu assay. The antioxidant capacity was measured by the DPPH and the ABTS radical scavenging assays. The radical-scavenging capacity was higher for pomegranate wine (statistically significant difference was observed for the DPPH assay) in agreement with its higher total phenolic content (383.19 ± 18.22 and 296.57 ± 25.23 mg gallic acid equivalents/100 mL for pomegranate and grape wine respectively). Customized HPLC-PDA-ESI-MSn and GC-MS methods were applied for the identification and chemical characterization of the phenolic compounds for both wines. Identification using LC-MS was based on their λmax (nm) and the characteristic fragments which derived from the sequential fragmentation in MS while GC-MS was based on commercial libraries and mass spectra of authentic standards. Eighty one different phenolic compounds were characterised by LC-MS and one hundred eight compounds by GC-MS after different chemical hydrolysis regimes. The study signifies the prior treatment with alkaline hydrolysis which had a considerable effect on the detection of phenolic compounds. The results showed that the combination of LC-MS and GC-MS methods allowed the detection of different compounds while results from both techniques are complementary and may confirm each other. Phytochemicals with proven biological activities including antimicrobial, antiviral and chemoprotective, have been identified mainly in pomegranate wines. Furthermore, a significant diversity between pomegranate and grape wines was observed, in terms of their phenolic content and antioxidant profiles indicating the-nutritive and health-promoting effects of pomegranate wine. |
Lantzouraki, D. Z.; Sinanoglou, V. J.; Zoumpoulakis, P.; Proestos, C. Characterization of the antioxidant and antiradical activity of pomegranate (Punicagranatum L.) extracts Journal Article In: Analytical Letters, 2015, (On line first (IF: 1.019)). BibTeX | Ετικέτες: Antioxidants, pomegranate @article{Lantzouraki2015b, |
Lantzouraki, D.; Sinanoglou, V. J; Zoumpoulakis, P.; Glamocilija, J.; Ciric, A.; Sokovic, M.; Heropoulos, G.; Proestos, C. Antiradical- antimicrobial activity and phenolic profile of pomegranate (Punicagranatum L.) juices from different cultivars: A comparative study Journal Article In: RSC Advances, vol. 5, pp. 2602-2614, 2015, ( (I F: 3.84)). Abstract | Links | BibTeX | Ετικέτες: Antiradical- antimicrobial activity, juice, phenolic profile, pomegranate @article{Lantzouraki2015, Pomegranate juice (PJ) constituents have shown to exhibit anticarcinogenic, antimicrobial, antioxidant and antiviral activities. In the present study, the concentration of phenolic compounds and the antiradical activity of PJs from the fruits of the two relatively new Greek cultivars “Persephone” and “Porphiroyeneti” were determined in comparison to the “Wonderful” cultivar. Total phenolic content and antiradical activity of the examined juices were found to vary in the same decreasing manner as follows: “Porphiroyeneti” > “Wonderful” > “Persephone”. The antimicrobial activity of PJs was also determined, showing equal or higher effect than commercial antimicrobial agents (streptomycin, ampicillin, bifonazole and ketoconazole). All tested extracts demonstrated noteworthy antibacterial activity with minimal inhibitory concentration ranging from 0.05 to 0.20 mg mL−1 and minimal bactericidal concentration ranging from 0.10 to 0.40 mg mL−1. Moreover, PJ extracts showed satisfactory fungistatic (0.05–0.2 mg mL−1) and fungicidal (0.1–0.3 mg mL−1) activity against all fungi tested. Among the cultivars tested, “Porphiroyeneti” showed slightly better antiradical and antimicrobial activity. In addition, a GC-MS methodology was developed for the determination of the phenolic profile of the extracts of PJ after different types of chemical hydrolysis. Finally, an HPLC-PDA-ESI-MSn analysis was conducted for the identification of the phenolic compounds in the extracts of PJ. In total, more than 30 non-anthocyanidinic and more than 20 anthocyanidinic compounds were identified. Our results confirm the functionality of pomegranate juices and the potential applications of PJ extracts towards novel products as food additives or preservatives. |